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Characteristics and Cryopreservation of Semen of Sex-Reversed Females of Salmonid Fish

Sylwia Judycka, Joanna Nynca, Piotr Hliwa, Andrzej Ciereszko

Int J Mol Sci. 2021 Jan; 22(2): 964. Published online 2021 Jan 19. doi: 10.3390/ijms22020964

PMCID:: PMC7835994

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Semen cryopreservation for the Mediterranean brown trout of the Biferno River (Molise-Italy): comparative study on the effects of basic extenders and cryoprotectants

Michele Di Iorio, Stefano Esposito, Giusy Rusco, Alessandra Roncarati, Marsia Miranda, Pier Paolo Gibertoni, Silvia Cerolini, Nicolaia Iaffaldano

Sci Rep. 2019; 9: 9703. Published online 2019 Jul 4. doi: 10.1038/s41598-019-45006-4

PMCID:: PMC6609640

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Identification of oxidatively modified proteins due to cryopreservation of carp semen

Agnieszka Mostek, Mariola Słowińska, Sylwia Judycka, Halina Karol, Andrzej Ciereszko, Mariola A Dietrich

J Anim Sci. 2018 Apr; 96(4): 1453–1465. Published online 2018 Mar 9. doi: 10.1093/jas/sky063

PMCID:: PMC6140970

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Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio L) semen

Mariola A. Dietrich, Andrzej Ciereszko

PLoS One. 2018; 13(3): e0192972. Published online 2018 Mar 22. doi: 10.1371/journal.pone.0192972

PMCID:: PMC5863941

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Semen collection by urethral catheterization and electro-ejaculation with different voltages, and the effect of holding temperature and cooling rate before cryopreservation on semen quality in the Japanese macaque (Macaca fuscata)

Noboru TAKAESU, Chihiro KANNO, Kosuke SUGIMOTO, Masashi NAGANO, Akihisa KANEKO, Yoriko INDO, Hiroo IMAI, Hirohisa HIRAI, Munehiro OKAMOTO, Mariko SASHIKA, Michito SHIMOZURU, Seiji KATAGIRI, Toshio TSUBOTA, Yojiro YANAGAWA

J Vet Med Sci. 2022 Mar; 84(3): 429–438. Published online 2022 Jan 24. doi: 10.1292/jvms.21-0590

PMCID:: PMC8983288

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Combined cryopreservation of canine ejaculates collected at a one‐hour interval increases semen doses for artificial insemination without negative effects on post‐thaw sperm characteristics

Dominik Lechner, Jörg Aurich, Sabine Schäfer‐Somi, Johannes Herbel, Christine Aurich

Reprod Domest Anim. 2021 Sep; 56(9): 1220–1226. Published online 2021 Jul 2. doi: 10.1111/rda.13980

PMCID:: PMC8518732

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Vapour fast freezing with low semen volumes can highly improve motility and viability or DNA quality of cryopreserved human spermatozoa

Valentina Arciero, Oumaima Ammar, Mario Maggi, Linda Vignozzi, Monica Muratori, Sara Dabizzi

Andrology. 2022 Sep; 10(6): 1123–1133. Published online 2022 Jun 27. doi: 10.1111/andr.13208

PMCID:: PMC9544568

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The cryopreservation process induces alterations in proteins associated with bull sperm quality: The equilibration process could be a probable critical control point

Ramasamy Arunkumar, Arumugam Kumaresan, Manish Kumar Sinha, Kamaraj Elango, John Peter Ebenezer Samuel King, Pradeep Nag, Thirumalaisamy Karuthadurai, Rubina Kumari Baithalu, Tushar Kumar Mohanty, Rakesh Kumar, Tirtha Kumar Datta

Front Endocrinol (Lausanne) 2022; 13: 1064956. Published online 2022 Dec 9. doi: 10.3389/fendo.2022.1064956

PMCID:: PMC9787546

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Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects

Lara Tamburrino, Giulia Traini, Arianna Marcellini, Linda Vignozzi, Elisabetta Baldi, Sara Marchiani

Int J Mol Sci. 2023 Mar; 24(5): 4656. Published online 2023 Feb 28. doi: 10.3390/ijms24054656

PMCID:: PMC10002855

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Egg yolk-free cryopreservation of bull semen

Muhammad Anzar, Kosala Rajapaksha, Lyle Boswall

PLoS One. 2019; 14(10): e0223977. Published online 2019 Oct 15. doi: 10.1371/journal.pone.0223977

PMCID:: PMC6793947

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Cryopreservation of Semen in Domestic Animals: A Review of Current Challenges, Applications, and Prospective Strategies

Mohsen Sharafi, Seyyed Mohsen Borghei-Rad, Maryam Hezavehei, Abdolhossein Shahverdi, James D. Benson

Animals (Basel) 2022 Dec; 12(23): 3271. Published online 2022 Nov 24. doi: 10.3390/ani12233271

PMCID:: PMC9739224

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Normal seminal plasma could preserve human spermatozoa against cryopreservation damages in Oligozoospermic patients

Fatemeh Eini, Maryam Azizi kutenaei, Maryam Hosseinzadeh Shirzeyli, Zeinolabedin Sharifian Dastjerdi, Mahmoud Omidi, Marefat Ghaffari Novin

BMC Mol Cell Biol. 2021; 22: 50. Published online 2021 Oct 5. doi: 10.1186/s12860-021-00390-6

PMCID:: PMC8491377

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Bacteriospermia and Sperm Quality of Cryopreserved Bull Semen Used in Artificial Insemination of Cows in South Wollo Zone, Ethiopia

Abadi Amare Reda, Gizat Almaw, Solomon Abreha, Wedajo Tadeg, Belege Tadesse

Vet Med Int. 2020; 2020: 2098315. Published online 2020 Jan 23. doi: 10.1155/2020/2098315

PMCID:: PMC7204317

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Different concentrations of cysteamine, ergothioneine, and serine modulate quality and fertilizing ability of cryopreserved chicken sperm

Pachara Thananurak, Napapach Chuaychu-noo, Aurore Thélie, Yupin Phasuk, Thevin Vongpralub, Elisabeth Blesbois

Poult Sci. 2020 Feb; 99(2): 1185–1198. Published online 2019 Nov 28. doi: 10.1016/j.psj.2019.10.040

PMCID:: PMC7587801

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Evaluation of Russian sturgeon (Acipenser gueldenstaedtii) Semen Quality and Semen Cryopreservation

Violeta Igna, Ada Telea, Tiana Florea, Roxana Popp, Adrian Grozea

Animals (Basel) 2022 Aug; 12(16): 2153. Published online 2022 Aug 22. doi: 10.3390/ani12162153

PMCID:: PMC9405347

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Involvement of Ca²⁺-ATPase in suppressing the appearance of bovine helically motile spermatozoa with intense force prior to cryopreservation

DURITAHALA, Mitsuhiro SAKASE, Hiroshi HARAYAMA

J Reprod Dev. 2022 Jun; 68(3): 181–189. Published online 2022 Mar 3. doi: 10.1262/jrd.2021-143

PMCID:: PMC9184823

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Extend the Survival of Human Sperm In Vitro in Non-Freezing Conditions: Damage Mechanisms, Preservation Technologies, and Clinical Applications

Qingyuan Cheng, Liman Li, Min Jiang, Bo Liu, Yang Xian, Shasha Liu, Xiao Liu, Wenrui Zhao, Fuping Li

Cells. 2022 Sep; 11(18): 2845. Published online 2022 Sep 12. doi: 10.3390/cells11182845

PMCID:: PMC9496714

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The Effect of Semen Cryopreservation Process on Metabolomic Profiles of Turkey Sperm as Assessed by NMR Analysis

Gianluca Paventi, Michele Di Iorio, Giusy Rusco, Anatoly P. Sobolev, Silvia Cerolini, Emanuele Antenucci, Mattia Spano, Luisa Mannina, Nicolaia Iaffaldano

Biology (Basel) 2022 May; 11(5): 642. Published online 2022 Apr 22. doi: 10.3390/biology11050642

PMCID:: PMC9138281

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Efficiency of Semen Cryopreservation in Stallions

Jörg Aurich, Juliane Kuhl, Alexander Tichy, Christine Aurich

Animals (Basel) 2020 Jun; 10(6): 1033. Published online 2020 Jun 13. doi: 10.3390/ani10061033

PMCID:: PMC7341245

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Effects of Slow Freezing and Vitrification of Human Semen on Post-Thaw Semen Quality and miRNA Expression

Rebeka Podgrajsek, Luka Bolha, Tjasa Pungert, Joze Pizem, Katerina Jazbec, Elvira Malicev, Martin Stimpfel

Int J Mol Sci. 2024 Apr; 25(8): 4157. Published online 2024 Apr 9. doi: 10.3390/ijms25084157

PMCID:: PMC11050687

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PMC Images search for cryopreserved semen【胚】

Figure 1

Absolute quantitative scatter plot for SARS coronavirus 2 genes detected using crystal digital PCR. Pooled semen samples were subjected to analyses. (A) N gene (grey: negative droplet, blue: positive droplet). (B) ORF-1ab gene (grey: negative droplet, green: positive droplet). (C) Human conserved region gene (grey: negative droplet, red: positive droplet). 1. Negative control; 2. Positive control; 3. Semen samples 1–10; 4. Semen samples 11–20; 5. Semen samples 21–30; 6. Semen samples 31–40; 7. Semen samples 41–50; 8. Semen samples 51–60; 9. Semen samples 61–70; 10. Semen samples 71–80; 11. Semen samples 81–90; 12. Semen samples 91–100.

Risks associated with cryopreserved semen in a human sperm bank during and after the COVID-19 pandemic

Reprod Biomed Online. 2021 Mar;42(3):589-594.
Figure 3.

Representative histograms displaying live and dead carp spermatozoa populations of fresh (A), fresh-diluted (B), and cryopreserved semen (C). Representative histograms showing ROS-positive and ROS-negative carp spermatozoa populations of fresh (D), fresh-diluted (E), and cryopreserved semen (F).

Identification of oxidatively modified proteins due to cryopreservation of carp semen

J Anim Sci. 2018 Apr;96(4):1453-1465.
Figure 3

A, Rate of usage (%) of cryopreserved semen for assisted reproductive treatment (ART). B, Types of cancer in oncology patients who used their cryopreserved semen for ART

Semen cryopreservation as an oncofertility treatment in 122 Japanese men with cancer: A decade‐long study

Reprod Med Biol. 2017 Oct;16(4):320-324.
Fig. 1.

Characteristics of motility of bovine cryopreserved spermatozoa. (A) Sperm motility patterns (helical movement and planar movement) of cryopreserved and non-cryopreserved semen which were diluted with phosphate-buffered saline containing 0.1% polyvinyl alcohol (PBS-PVA). (B) Typical examples of the microscopic images (two sequential frames which were captured at a frame rate of 100 Hz) of helically motile spermatozoa and planarly motile spermatozoa of the cryopreserved semen which was diluted with PBS-PVA; the flagellum of each spermatozoon (indicated with a white arrow) is highlighted with a red line to readily recognize its shape. (C) The average relative velocity of helically and planarly motile spermatozoa of the cryopreserved semen which was diluted with PBS-PVA. (D) Sperm motility patterns of cryopreserved semen which was diluted with phosphate-buffered saline containing 1 or 2.5% polyvinylpyrrolidone (PBS-PVP); values with different letters (A versus B, C versus D, a versus b, c versus d) are significantly different (P < 0.05). (E) The average relative velocity of helically and planarly motile spermatozoa of the cryopreserved semen which was diluted with PBS-PVP.

Involvement of Ca2+-ATPase in suppressing the appearance of bovine helically motile spermatozoa with intense force prior to cryopreservation

J Reprod Dev. 2022 Jun;68(3):181-189.